Western Blot:Article Title: HBO1 determines SMAD action in pluripotency and mesendoderm specification
Article Snippet: .. List of antibodies used in this study Name of Antibody Company (Cat. No.) Application HRP-Conjugated GAPDH Monoclonal Antibody Proteintech (HRP-60004) WB Rabbit anti-MYST2 (HBO1) Cell Signaling Technology (58418S) WB/IF Mouse anti-OCT-3/4 Santa Cruz Biotechnology (sc-5279) WB Rabbit anti-NANOG Cell Signaling Technology (3580S) WB Rabbit anti-Sox2 Cell Signaling Technology (23064S) WB/IF Mouse anti-SOX2 R&D (mab2018) WB/IF Rabbit anti-HIST3H3 (H3) Abcam (ab1791) WB Rabbit anti-Histone H3 (acetyl K23) Abcam (ab177275) WB Rabbit anti-Histone H3 (acetyl K14) Cell Signaling Technology (7627S) WB Rabbit anti-Histone H4 (acetyl K5) Cell Signaling Technology (8346T) WB Rabbit anti-Histone H4 (tri methyl K12) Cell Signaling Technology (8346T) WB mouse Monoclonal ANTI-FLAG Sigma (F1804) WB/FACS/Co-IP/CUT-Tag mouse anti-HA Sigma (H3663) Co-IP Rabbit anti-SMAD4 Cell Signaling Technology (46535) CUT-Tag Rabbit anti-TUJ1 Genetex (GTX130245) IF Rabbit anti-MAP2 Cell Signaling Technology (8707) IF Mouse anti-NES Cell Signaling Technology (33475) IF Rabbit anti-GATA3 Cell Signaling Technology (5852) IF Goat anti-T R&D (AF2085) IF Goat anti-SOX17 R&D (AF1924) IF Rabbit anti-GATA6 Cell Signaling Technology (5851T) IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific (A-11004) IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific (A-11011) IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-11008) IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-11001) FACS Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific (A-21447) IF Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-21206) IF Mouse anti-PAX6 BD Biosciences (561664) FACS Anti-Brachyury-FITC Antibody Sigma (FCMAB302F) FACS Alexa Fluor®488 Mouse IgG2a, κ Isotype control BD biosciences (558055) FACS ..
Co-Immunoprecipitation Assay:Article Title: HBO1 determines SMAD action in pluripotency and mesendoderm specification
Article Snippet: .. List of antibodies used in this study Name of Antibody Company (Cat. No.) Application HRP-Conjugated GAPDH Monoclonal Antibody Proteintech (HRP-60004) WB Rabbit anti-MYST2 (HBO1) Cell Signaling Technology (58418S) WB/IF Mouse anti-OCT-3/4 Santa Cruz Biotechnology (sc-5279) WB Rabbit anti-NANOG Cell Signaling Technology (3580S) WB Rabbit anti-Sox2 Cell Signaling Technology (23064S) WB/IF Mouse anti-SOX2 R&D (mab2018) WB/IF Rabbit anti-HIST3H3 (H3) Abcam (ab1791) WB Rabbit anti-Histone H3 (acetyl K23) Abcam (ab177275) WB Rabbit anti-Histone H3 (acetyl K14) Cell Signaling Technology (7627S) WB Rabbit anti-Histone H4 (acetyl K5) Cell Signaling Technology (8346T) WB Rabbit anti-Histone H4 (tri methyl K12) Cell Signaling Technology (8346T) WB mouse Monoclonal ANTI-FLAG Sigma (F1804) WB/FACS/Co-IP/CUT-Tag mouse anti-HA Sigma (H3663) Co-IP Rabbit anti-SMAD4 Cell Signaling Technology (46535) CUT-Tag Rabbit anti-TUJ1 Genetex (GTX130245) IF Rabbit anti-MAP2 Cell Signaling Technology (8707) IF Mouse anti-NES Cell Signaling Technology (33475) IF Rabbit anti-GATA3 Cell Signaling Technology (5852) IF Goat anti-T R&D (AF2085) IF Goat anti-SOX17 R&D (AF1924) IF Rabbit anti-GATA6 Cell Signaling Technology (5851T) IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific (A-11004) IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific (A-11011) IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-11008) IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-11001) FACS Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific (A-21447) IF Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-21206) IF Mouse anti-PAX6 BD Biosciences (561664) FACS Anti-Brachyury-FITC Antibody Sigma (FCMAB302F) FACS Alexa Fluor®488 Mouse IgG2a, κ Isotype control BD biosciences (558055) FACS ..
FACS:Article Title: HBO1 determines SMAD action in pluripotency and mesendoderm specification
Article Snippet: .. List of antibodies used in this study Name of Antibody Company (Cat. No.) Application HRP-Conjugated GAPDH Monoclonal Antibody Proteintech (HRP-60004) WB Rabbit anti-MYST2 (HBO1) Cell Signaling Technology (58418S) WB/IF Mouse anti-OCT-3/4 Santa Cruz Biotechnology (sc-5279) WB Rabbit anti-NANOG Cell Signaling Technology (3580S) WB Rabbit anti-Sox2 Cell Signaling Technology (23064S) WB/IF Mouse anti-SOX2 R&D (mab2018) WB/IF Rabbit anti-HIST3H3 (H3) Abcam (ab1791) WB Rabbit anti-Histone H3 (acetyl K23) Abcam (ab177275) WB Rabbit anti-Histone H3 (acetyl K14) Cell Signaling Technology (7627S) WB Rabbit anti-Histone H4 (acetyl K5) Cell Signaling Technology (8346T) WB Rabbit anti-Histone H4 (tri methyl K12) Cell Signaling Technology (8346T) WB mouse Monoclonal ANTI-FLAG Sigma (F1804) WB/FACS/Co-IP/CUT-Tag mouse anti-HA Sigma (H3663) Co-IP Rabbit anti-SMAD4 Cell Signaling Technology (46535) CUT-Tag Rabbit anti-TUJ1 Genetex (GTX130245) IF Rabbit anti-MAP2 Cell Signaling Technology (8707) IF Mouse anti-NES Cell Signaling Technology (33475) IF Rabbit anti-GATA3 Cell Signaling Technology (5852) IF Goat anti-T R&D (AF2085) IF Goat anti-SOX17 R&D (AF1924) IF Rabbit anti-GATA6 Cell Signaling Technology (5851T) IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific (A-11004) IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific (A-11011) IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-11008) IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-11001) FACS Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific (A-21447) IF Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-21206) IF Mouse anti-PAX6 BD Biosciences (561664) FACS Anti-Brachyury-FITC Antibody Sigma (FCMAB302F) FACS Alexa Fluor®488 Mouse IgG2a, κ Isotype control BD biosciences (558055) FACS ..
Control:Article Title: HBO1 determines SMAD action in pluripotency and mesendoderm specification
Article Snippet: .. List of antibodies used in this study Name of Antibody Company (Cat. No.) Application HRP-Conjugated GAPDH Monoclonal Antibody Proteintech (HRP-60004) WB Rabbit anti-MYST2 (HBO1) Cell Signaling Technology (58418S) WB/IF Mouse anti-OCT-3/4 Santa Cruz Biotechnology (sc-5279) WB Rabbit anti-NANOG Cell Signaling Technology (3580S) WB Rabbit anti-Sox2 Cell Signaling Technology (23064S) WB/IF Mouse anti-SOX2 R&D (mab2018) WB/IF Rabbit anti-HIST3H3 (H3) Abcam (ab1791) WB Rabbit anti-Histone H3 (acetyl K23) Abcam (ab177275) WB Rabbit anti-Histone H3 (acetyl K14) Cell Signaling Technology (7627S) WB Rabbit anti-Histone H4 (acetyl K5) Cell Signaling Technology (8346T) WB Rabbit anti-Histone H4 (tri methyl K12) Cell Signaling Technology (8346T) WB mouse Monoclonal ANTI-FLAG Sigma (F1804) WB/FACS/Co-IP/CUT-Tag mouse anti-HA Sigma (H3663) Co-IP Rabbit anti-SMAD4 Cell Signaling Technology (46535) CUT-Tag Rabbit anti-TUJ1 Genetex (GTX130245) IF Rabbit anti-MAP2 Cell Signaling Technology (8707) IF Mouse anti-NES Cell Signaling Technology (33475) IF Rabbit anti-GATA3 Cell Signaling Technology (5852) IF Goat anti-T R&D (AF2085) IF Goat anti-SOX17 R&D (AF1924) IF Rabbit anti-GATA6 Cell Signaling Technology (5851T) IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific (A-11004) IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific (A-11011) IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-11008) IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-11001) FACS Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific (A-21447) IF Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific (A-21206) IF Mouse anti-PAX6 BD Biosciences (561664) FACS Anti-Brachyury-FITC Antibody Sigma (FCMAB302F) FACS Alexa Fluor®488 Mouse IgG2a, κ Isotype control BD biosciences (558055) FACS ..
Recombinant:Article Title: Post-replicative initial expression of PAX6 during neuroectoderm differentiation.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models H9 embryonic stem cell WiCell Research Institute WA09 Recombinant DNA Plasmid FUCCI Addgene Cat #86849 Plasmid LentiCRISPR v2 Addgene Cat #52961 Plasmid pMD2.G Addgene Cat #12259 Plasmid psPAX2 Addgene Cat #12260 Plasmid PAX6 promoterdriven miRFP680 This study / Reagent/resource Reference or source Identifier or catalog number Antibodies Rabbit anti-PAX6 Cell Signaling Technology Cat #60433 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig Mouse anti-OCT3/4 Santa Cruz Biotechnology Cat #sc-5279 Goat anti-NANOG R&D Systems Cat AF1997 Mouse anti-TUJ1 R&D Systems Cat #MAB1195 Rabbit anti-SOX2 Cell Signaling Technology Cat #3579S Rabbit anti-PAX6 Invitrogen Cat #42-6600 Alexa Fluor® 647 Mouse anti-Oct3/4 BD Biosciences Cat #560307 Donkey anti-rabbit IgG (H+ L) antibody Alexa FluorTM 488 Invitrogen Cat #A32790 Donkey anti-rabbit IgG (H+ L) antibody with Alexa FluorTM 647 Invitrogen Cat #A32795 Donkey anti-rabbit IgG (H+ L) antibody with Alexa FluorTM 594 Invitrogen Cat #A32754 Goat Anti-Rabbit IgG H&L Vazyme Cat #Ab206-01 Figure 7. ..
Plasmid Preparation:Article Title: Post-replicative initial expression of PAX6 during neuroectoderm differentiation.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models H9 embryonic stem cell WiCell Research Institute WA09 Recombinant DNA Plasmid FUCCI Addgene Cat #86849 Plasmid LentiCRISPR v2 Addgene Cat #52961 Plasmid pMD2.G Addgene Cat #12259 Plasmid psPAX2 Addgene Cat #12260 Plasmid PAX6 promoterdriven miRFP680 This study / Reagent/resource Reference or source Identifier or catalog number Antibodies Rabbit anti-PAX6 Cell Signaling Technology Cat #60433 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig Mouse anti-OCT3/4 Santa Cruz Biotechnology Cat #sc-5279 Goat anti-NANOG R&D Systems Cat AF1997 Mouse anti-TUJ1 R&D Systems Cat #MAB1195 Rabbit anti-SOX2 Cell Signaling Technology Cat #3579S Rabbit anti-PAX6 Invitrogen Cat #42-6600 Alexa Fluor® 647 Mouse anti-Oct3/4 BD Biosciences Cat #560307 Donkey anti-rabbit IgG (H+ L) antibody Alexa FluorTM 488 Invitrogen Cat #A32790 Donkey anti-rabbit IgG (H+ L) antibody with Alexa FluorTM 647 Invitrogen Cat #A32795 Donkey anti-rabbit IgG (H+ L) antibody with Alexa FluorTM 594 Invitrogen Cat #A32754 Goat Anti-Rabbit IgG H&L Vazyme Cat #Ab206-01 Figure 7. ..
Immunohistochemistry:Article Title: Single cell analysis of olfactory neurogenesis and differentiation in adult humans
Article Snippet: .. Case Number Age (years) Gender Experimental Use Clinical History 1 41 F IHC, scRNA-seq Pituitary adenoma 2 52 M IHC, scRNA-seq Esthesioneuroblastoma (contralateral, intracranial) 3 44 F IHC, scRNA-seq Pituitary adenoma 4 50 F scRNA-seq Pituitary adenoma 5 39 F IHC Pituitary adenoma 6 47 M IHC Pituitary adenoma 7 23 F IHC Pituitary adenoma REAGENT SOURCE IDENTIFIER LOT# DILUTION Mouse anti-TUJ1 BioLegend Cat# 801201; AB_2313773 B264428 1:500 Rat anti-SOX2 eBioscience Cat# 14-9811-82; AB_11219471 2023691 1:50 Rabbit anti-SOX2 Cell Signaling Technology Cat# 3579; AB_2195767 01/2014 Lot:5 1:50 Rabbit anti-KRT5 Abcam Cat# ab52635; AB_869890 GR3198825-2 1:1000 Rabbit anti-LHX2 Millipore Cat# ABE1402; AB_2722523 3030529 1:500 Rabbit anti-GNG13 Sigma Cat# HPA046272; AB_10960062 R42082 1:150 Rabbit anti-Ki67 Abcam Cat# ab15580; AB_443209 GR3293897-3 1:250 Rabbit anti- Doublecortin Cell Signaling Technology Cat# 4604; AB_561007 08/2018 Lot:5 1:200 Mouse anti-OMP Santa Cruz Biotechnology Cat# sc-365818; AB_10842164 E1117 1:500 Goat anti-OLIG2 R&D Systems Cat# AF2418; AB_2157554 UPA0717121 1:500 Mouse anti-TP63 Santa Cruz Biotechnology Cat# sc-5301; AB_628093 D2519 1:100 AlexaFluor 594 Goat anti-Mouse Jackson ImmunoResearch Cat# 115-585-146; AB_2338881 109495 1:100 AlexaFluor 488 Donkey anti-Mouse Jackson ImmunoResearch Cat# 715-545-150; AB_2340846 113699 1:100 AlexaFluor 594 Goat anti-Rabbit Jackson ImmunoResearch Cat# 111-585-144; AB_2307325 106684 1:100 AlexaFluor 488 Donkey anti-Rat Jackson ImmunoResearch Cat# 712-545-150; AB_2340683 113383 1:100 AlexFluor 488 Donkey anti-Goat Jackson ImmunoResearch Cat# 705-545-147; AB_2336933 115940 1:100 ..
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![Figure 5. Functional in vitro validation of SOX2 C terminus-imposed translational adaptations (A) Relative changes in glucose uptake in response to SOX2 (fragment) induction in T47D cells. Indicated are MFI shifts at l = 488 nm (D mean 488 nm) reflecting SOX2 (fragment)-induced changes to the incorporation rate of glucose uptake reporter 2-NBDG. The endogenous uptake rate into DOX-exposed but empty control cells is set to baseline. Dots indicate mean intensity shift in 4 independent experiments. (B) Representative example of a metabolic Seahorse analysis indicating aggravated sugar consumption (followed as extracellular acidification rate [ECAR]) in starved T47D cells expressing either full-length SOX2 (1–317, red) or an NES/C-terminal segment thereof (180–317, green) vs. equally treated empty control cells (black) or T47D cells expressing the SOX2 N terminus (1–179, blue). Dots reflect means of 4 or more technical replicates.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_8083/pm39368083/pm39368083__page12_image1.jpg)
